Evaluation of the effects of 5-fluorouracil and cyclophosphamide on Lathyrus sativus L.

Abstract

The effect of the selected drugs on radicle length, colchicine induces polyploidy and in vitro callus growth has been studied (concentration used: 5-Fluorouracil—0.01, 0.1, 1 and 10 mM; Cyclophosphamide—1, 10, 20 and 30 mM). The biochemical studies on superoxide dismutase, catalase and lipid peroxidase activity also studied. Radicle length, polyploidy percentage and callus growth decrease in both the drugs in a dose-dependent manner. The SOD, catalase and LP activity decrease with the increase in drug concentration except for low dose (for 5FU—0.01 mM and 1 mM for CP). Induced polyploidy (Control B) than water germinated seedling (Control A) shows higher enzyme activity but a decrease in the increased dose of drugs.