Construction and validation of a multi-epitope in silico vaccine model for lymphatic filariasis by targeting Brugia malayi: a reverse vaccinology approach

Abstract

Prediction of T cell epitopes and prioritization In this study, CTL epitopes were predicted using the IEDB MHC-I processing tool, whereas HTL epitopes were predicted using the IEDB MHC-II Binding Predictions tool and both types of T cell epitopes were predicted by the SMM approach against a set of human reference HLAs with adjusted IC50 value less than 50 nM. A total of 15 CTL epitopes and 3 HTL epitopes were shortlisted for eight selected antigenic proteins by applying several immunological filters as shown in Tables 1 and 2. All of the optimal epitopes, as well as their antigenicity, positioning and binding alleles, are documented in Tables 3 and 4. In addition, for the best possible epitopes, the corresponding alleles which have a moderate binding affinity (IC50 < 500 nM) were also documented.