Browsing by Author El Shebini, Salwa Mostafa

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  • Authors: El Shebini, Salwa Mostafa;  Advisor: -;  Participants: Fouad, Suzanne; Abdel-Moaty, Maha; Ahmed, Nihad Hassan; Hussein, Ahmed Mohamed Saied; Essa, Hend Abass; Tapozada, Salwa Tawfic (2020)

  • B. subtilis MK1 α-amylase was successfully immobilized on chitosan-magnetic nano-particles using a method combining the advantages of both physical adsorption and covalent binding. The beads were chemically modified using polyethyleneimine (PEI) followed by glutaraldehyde (GA). Aminated beads by (PEI), activated beads by (GA), and immobilized enzyme on activated beads were characterized using FTIR. Morphological examinations of the beads surface before and after conjugation with the α-amylase enzyme were carried out using scanning electron microscope (SEM). Chemical modification parameters of the beads were optimized using response surface methodology based on central composite design...