BBAuthors: Hassan, M.E.; Advisor: -; Participants: Yang, Q.; Xiao, Z. (2019)
The immobilized enzyme shows enhancement in temperature profile as the optimum temperature for the free enzyme was 60 °C, and it becomes 60–80 °C that means broader range and also shows stability in acidic conditions more than free enzyme. The apparent Km of immobilized enzyme, 147.46 mM, becomes higher than Km of the free one, 110 mM and the maximum reaction velocity (Vmax) values for the immobilized enzyme decreased from 2.28 to 1.11 μmol min−1. The immobilized enzyme can be reused and kept its activity (100%) till 11 successive cycles. The immobilization process steps were characterized by FTIR and SEM. 
