BBAuthors: Tamboli, Ashpak Mubarak; Advisor: -; Participants: Wadkar, Kiran Anna (2022)
The presence of various types of phytoconstituents of the extract was detected by phytochemical screening. The leaves of Convolvulus pluricaulis were found to contain alkaloids, flavonoids, tannins, sterols and carbohydrate.
Cytotoxic activity of Convolvulus pluricaulis leaves extracts on Hep G2 and Line-L-929 cell lines was evaluated by MTT assay. MTT assay is a colorimetric assay that assesses mitochondrial succinate dehydrogenase's reduction of yellow 3-(4, 5 dimethylthiazole-2-yl)2,5-diphenyltetrazolium bromide (MTT). The MTT enters into mitochondria of cell and reduces to an insoluble colored complex (dark purple) formazan. Decline of MTT occurs in the metabolically active cel... 
