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Title: | Early-pandemic wastewater surveillance of SARS-CoV-2 in Southern Nevada: Methodology, occurrence, and incidence/prevalence considerations |
Authors: | Gerrity, D. |
Participants: | Papp, K. Stoker, M. Sims, A. Frehner, W. |
Issue Date: | 2021 |
Series/Report no.: | Water Research X, Volume 10, 1 January 2021, 100086 |
Abstract: | The current study aimed to compare the performance of virus recovery and detection methods, detect and quantify SARS-CoV-2 genetic material in two Southern Nevada sewersheds from March–May 2020, and better understand the potential link between COVID-19 incidence/prevalence and wastewater concentrations of SARS-CoV-2 RNA. SARS-CoV-2 surrogate recovery (0.34%–55%) and equivalent sample volume (0.1 mL–1 L) differed between methods and target water matrices, ultimately impacting method sensitivity and reported concentrations. Composite sampling of influent and primary effluent resulted in a ∼10-fold increase in concentration relative to corresponding grab primary effluent samples, presumably highlighting diurnal variability in SARS-CoV-2 signal. Detection and quantification of four SARS-CoV-2 genetic markers (up to ∼106 gene copies per liter), along with ratios of SARS-CoV-2 to pepper mild mottle virus (PMMoV), exhibited comparability with public health data for two sewersheds in an early phase of the pandemic. Finally, a wastewater model informed by fecal shedding rates highlighted the potential significance of new cases (i.e., incidence rather than prevalence) when interpreting wastewater surveillance data. |
URI: | http://tailieuso.tlu.edu.vn/handle/DHTL/10510 |
Source: | https://www.sciencedirect.com/science/article/pii/S2589914720300463 |
ISSN: | 2589-9147 |
Appears in Collections: | Tài liệu mở |
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