Phytochemical analysis of Eucalyptus camaldulensis leaves extracts and testing its antimicrobial and schistosomicidal activities

Abstract

Organic solvent extracts from Eucalyptus camaldulensis leaves were assessed for their antimicrobial activity. Among these extracts, ethyl acetate (EtOAc) and water extracts showed the highest antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger. EtOAc extract was further subjected to vacuum liquid chromatography (VLC) technique for the isolation of its polyphenolic ingredients. VLC yielded 13 fractions (1–13) that were assayed for their antimicrobial activities. Six of these fractions, namely 4, 5, 6, 7, 8, and 9, showed antimicrobial activity against all the tested microbes except the fungus Aspergillus niger. Fractions 5 and 6 having considerably the highest antimicrobial activity with inhibition zones ranged from 5 to 14 mm.