Antimicrobial, antidermatophytic, and cytotoxic activities from Streptomyces sp. MER4 isolated from Egyptian local environment

Abstract

Streptomyces sp. MER4 (accession no. KM099241) was isolated from the pyramid region, Giza, Egypt. This strain was previously mentioned for its ability to produce antidermatophytic bioactive metabolites. Scale-up fermentation and fractionation of the extract has been established using different solvents. The ethanol fraction exhibited a considerable antidermatophytic effect (19.8, 21.2, and 20.3 mm for Trichophyton mentagrophyes, Microsporum canis, and Microsporum gypseum, respectively), antimicrobial (10, 8, 7, 9, and 9 mm for Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, Bacillus subtilis, and Aspergillus niger, respectively), and cytotoxic activity (inhibition of 78.48%). Further purification of the ethanol fraction was done, and one promising compound was produced. This compound was intensely characterized and elucidated by studying its spectral date including nuclear magnetic resonance (NMR) and liquid chromatography mass spectroscopy (LC/MS). The produced compound was identified as JBIR-58 (salicylamide) derivative compound.