Evaluation of the benzo[a]anthracene biodegradation by animal bioassays

Abstract

B. amyloliquefaciens was exposed to different doses of γ radiation (0.5, 1.0, 1.5, and 2.0 kGy) kGy. The colonies for the wild strain and its variants obtained after radiation were counted. The final counts for variant 3 (V3), variant 4 (V4), and variant 5 (V5) have been increased from its initial count by (0.3, 0.48, and 0.1 log cycle) respectively at 1 mg/100 ml (BAA). For animal bioassay, male mice were divided into seven groups; control group received vehicle only, groups II and III were injected with 5 and 10 mg/kg b.wt (BAA) respectively, and groups IV, V, VI, and VII were injected with the residues of BAA after biodegradation with wild type, V3, V4, and V5 of B. amyloliquefaciens respectively.