Development of DNA-based species identification and barcoding of three important timbers

Abstract

Among the three selected markers, only two markers (rbcL and trnH-ITS) were having potential to amplify and discriminate the intra- and interspecific regions of selected timbers and would have been used to get barcodes for each species. Additionally, rbcL (90–96%) has a higher discrimination dominance over trnH-ITS (80–86%). From the barcoding results, it was found that the T. grandis and D. latifolia samples that belong to the Institute of Wood Science and Technology Bangalore are genetically more related to Dharwad than Hassan region in Karnataka.