Generation of CRISPR-cas9 construct for knockout of genes encoding chromatin-associated proteins

Abstract

From TrxG, ISWI and Acf were selected, and from PcG, Pc and Psc were selected. Three pAc-sgRNA-Cas9-puro-vector constructs for ISWI gene, one pAc-sgRNA-cas9-puro-vector construct for Pc, gene and two pAc-sgRNA-cas9-puro-vector constructs for each of the Acf and Psc gene were generated. These constructs were confirmed by PCR and sequencing. In the future, these constructs will be used to study the role of their respective target genes in chromatin organization.