Regeneration of rice somaclons tolerant to high level of abscisic acid and their characterization via RAPD markers

Abstract

The direct in vitro selection method indicated that the callus weight of the two cultivars was significantly decreased in response to increasing ABA concentrations. The shoot formation revealed significant differences among ABA concentrations and highly significant for interaction between rice cultivars and ABA concentrations. The percentage of shoot formation either increased or decreased with increasing ABA levels in rice cultivars. However, Giza 178 cultivar was slightly stable for shoot formation than the cultivar Sakha 102. The obtained results for stepwise selection method indicated also that the shoot formation was obtained till 6 mg/L ABA. The results of the RAPD analysis indicated that the rice cultivars and their somaclons were classified into tow clusters. The polymorphic information content (PIC) per primer ranged from 0.715 to 0.906 with an average of 0.860. The results of UPGMA clustering were consistent with those of the PCoA.