Molecular genetic studies on abiotic stress resistance in sorghum entries through using half diallel analysis and inter-simple sequence repeat (ISSR) markers

Abstract

The recent genotypes were estimated through some physiological parameters related to water stress tolerance in sorghum; besides that, eight inter-simple sequence repeat (ISSR) primers were used to identify among the five sorghum parents and the highest five crosses resistance to water deficit conditions depending on the data calculated from all studied traits under both conditions. The following genotypes P1, P2, P3, P1 × P2, P1 × P3, P2 × P3, P2 × P4, and P3 × P4 confirmed high resistance to water deficit conditions under the drought treatment compared with the control. This high resistance was affirmed through the calculated data for all studied traits. The ISSR profile analysis showed 151 fragments as taxonomic divisions among the ten sorghum genotypes (38 of them were monomorphic and 113 polymorphic with 74.83% polymorphism).