Using high frequency oligonucleotides-targeting active gene (HFO-TAG) markers for genetic evaluation among genotypes (Cucurbita pepo L. and C. maxima L.)

Abstract

A total of 107 alleles were detected by the 15 HFO-TAG markers, an average of 7.133 alleles per primer. Polymorphisms were found in 102 alleles, an average of 6.866 alleles per primer. The PIC values measured from all of the HFO-TAG markers were high, and ranged from 0.8940 to 0.7225, with an average 0.8212 per marker. The results of the cluster analysis of pumpkin genotypes were separated into seven groups according to the collection region.