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Title: | In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv. “Starfighter” plants |
Authors: | Youssef, Nora M. |
Participants: | Shaaban, Shimaa A. Ghareeb, Zenna Fawzia Taha, Lobna S. |
Issue Date: | 2019 |
Series/Report no.: | Bulletin of the National Research Centre, Volume 43 (2019), Article number: 211 |
Abstract: | For direct shoot organogenesis, culturing leaf explant on MS culture medium containing TDZ (0.5 mg/l) + 2,4-D (10 mg/l) led to the highest number of shootlets after 1 month. For indirect micropropagation, MS was supplemented with 2ip (0.5 mg/l) + picloram (5 mg/l)-induced callus tissues which were differentiated to embryos then weak shoots after 3 months. For more proliferation, the highest numbers of shootlets, leaves, and bulb scales were observed on MS culture medium supplemented with 1.0 mg/l of BA plus 0.2 mg/l NAA. Using 60 g/l of sucrose plus 3 or 6 mg/l of paclobutrazol led to the highest number of bulblets/explant for the third subculture. The highest bulblet diameters were recorded with 120 g/l sucrose plus 6 or 3 mg/l paclobutrazol. |
URI: | http://tailieuso.tlu.edu.vn/handle/DHTL/11122 |
Source: | https://bnrc.springeropen.com/articles/10.1186/s42269-019-0246-z |
ISSN: | 2522-8307 |
Appears in Collections: | Tài liệu mở |
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