The influence of He-Ne laser on agro-morphological criteria, ISSR marker and SDS-PAGE of Moringa oleifera

Abstract

Most agro-morphological criteria increased especially with wetted seed group pre-laser treatments (25 min). SDS-PAGE and ISSR profiles showed changes which include the absence of some bands and the appearance of few novel bands. ISSR markers showed a change in band number in all investigated samples with a total number of 172 bands. The polymorphic bands were 91 bands with 19 unique bands. The average mean percentage of polymorphism was recorded 52.91% at samples treated with a laser at different times. Wetted seed treatment for 25 min recorded 40 bands as new bands. Genetic template stability (GTS) values were recorded in all treatments. The lowest GTS % was recorded 76.74 at 25 min (wetted seeds) while highest GTS % was recorded 83.72 at 25 min (dry seeds). According to the data of GTS, produced by laser treatment for 25 and 45 min for wetted and dry seeds, respectively, it is more effective on genome stability than other treatments.