Biochemical characterization of micropropagated Ceratonia siliqua L. under effect of growth regulators and light quality

Abstract

MS culture medium supplemented with BA produced the highest shootlets number/explants. For light quality, the data showed that green light increased shootlet number/explants, fresh and dry weights. However, yellow light increased shootlet length and leaves number and also caused significant increase in total phenols, flavonoids, and antioxidant capacity (DPPH) as compared to other lights. HPLC analysis showed that yellow light caused the highest accumulation of total phenolic and catechin that were the highest accumulated compounds (509 μg/ml) followed by that were accumulated under green light (412.68 μg/ml). Shootlets grown under white light (control) gave the highest accumulation of p-coumaric acid, rosmarinic acid, and cinnamic acid. Shootlets grown under blue light gave the highest accumulation of gentisic acid and syringic acid. Red light caused the highest accumulation of vanillic acid. However, white light accumulated the highest amount of flavonoid compounds comparing with other light qualities.