Comparison of conventional and automated freezing methods on PB2 rooster semen cryopreserved with glycerol and dimethylsulfoxide tris coconut-water extender

Abstract

At 8% glycerol concentration, FP1 has significantly (P > 0.05) the highest percentage motility (73.33%) compared to FPs 2, 3, and 4 (56.68, 50.00, and 23.33% respectively). At 10% glycerol, FP2 had the highest motility (48.33%) and HOST (64.00%). At 15% glycerol, FP4 (fast freezing) had the highest percentage motility (71.67%), viability (76.33%), and HOST (71.67%). At 8%, DMSO concentrations FP4 had the highest significant (P > 0.05) motility, viability, and HOST. Ten percent and 15% DMSO revealed no significant (P > 0.05) difference in most parameters among all the FPS. DMSO performed better than glycerol irrespective of concentrations in all FPS on most parameters evaluated.