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Browsing by Author Gabr, Ahmed Mohamed Magdy

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  • Optimization of in vitro culture conditions affecting propagation of mulberry plant

    • Authors: Taha, Hussein;  Advisor: -;  Participants: Ghazy, Usama Mohamed; Gabr, Ahmed Mohamed Magdy; EL-Kazzaz, Ahmed Ahmed Ahmed; Ahmed, Eman Abdel Mottaleb Mahmoud; Haggag, Karima Mohamed (2020)

  • A simple and efficient protocol for in vitro propagation of different varieties of Morus alba (yue 11, sha 2x lun 109, morittina, kokuso 27, and kantava 2) was established. Sterilization of shoot tips and auxiliary buds with 0.2% of mercuric chloride (MC) for 10 min was the best to get sterilized and survival explants. In vitro mulberry multiplication of yue 11, sha 2x lun 109, morittina, kokuso 27, and kantava auxiliary buds cultured on the MS medium fortified with 1.5 mg/l BA gave the best results of the multiplication rates, maximum number of shoots/explant, and highest shoot length (cm), respectively. Moreover, culturing of derived mulberry shoots on the MS medium supplemented wit...
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  • Rutin accumulation in gardenia calli cultures as a response to phenyl alanine and salicylic acid

    • Authors: El-Ashry, Amal Abd El-Latif;  Advisor: -;  Participants: Gabr, Ahmed Mohamed Magdy; Arafa, Nermeen Mohamed; El-Bahr, Mohamed Kamal (2019)

  • The results showed that the highest value of total phenols (9.53 ± 0.55 mg GAE/g DW of callus) was recorded with 5 mg/l phenylalanine (PA) at the third harvest. While the highest value of total flavonoids (0.358 ± 0.002 mg QE/g DW of callus) was achieved using 2 mg/l P.A. at the third harvest. By using HPLC, it was found that the maximum rutin content was recorded with 3 mg/l PA at the third harvest (429 μg/g DW). This study showed that rutin and cinnamic acids were the major compounds detected with all treatments and during the three harvest phases of gardenia calli cultures. As well as results declared that the decreasing of cinnamic acid was correlated with the increasing of rutin ...