BBAuthors: El-Ashry, Amal Abd El-Latif; Advisor: -; Participants: Gabr, Ahmed Mohamed Magdy; Arafa, Nermeen Mohamed; El-Bahr, Mohamed Kamal (2019)
The results showed that the highest value of total phenols (9.53 ± 0.55 mg GAE/g DW of callus) was recorded with 5 mg/l phenylalanine (PA) at the third harvest. While the highest value of total flavonoids (0.358 ± 0.002 mg QE/g DW of callus) was achieved using 2 mg/l P.A. at the third harvest. By using HPLC, it was found that the maximum rutin content was recorded with 3 mg/l PA at the third harvest (429 μg/g DW). This study showed that rutin and cinnamic acids were the major compounds detected with all treatments and during the three harvest phases of gardenia calli cultures. As well as results declared that the decreasing of cinnamic acid was correlated with the increasing of rutin ... 
