Biotechnological and chemical analysis of Egyptian Diospyros kaki L. cv. Costata grown in Egypt

Abstract

The highest amounts of scopoletin 57.08, 26.42, and 25.30 (μg/g DW) were detected using reversed phase of high-performance liquid chromatography (RP-HPLC) in leaves extract of intact plant followed by regenerated and calli cultures of internod explants, respectively. This study is the first record for in vitro propagation and production of secondary metabolites from Diospyros kaki L. using biotechnology techniques. Chemical anaysis were carried out using HPLC technique.